Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Oct;67(2):510-6.
doi: 10.1073/pnas.67.2.510.

Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1

S N Cohen, C A Miller

Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1

S N Cohen et al. Proc Natl Acad Sci U S A. 1970 Oct.

Abstract

A covalently-closed circular DNA species, banding at a buoyant density of rho = 1.709 g/cm(3) in CsCl, has been identified in antibiotic-sensitive colonies of E. coli strain AB2463 (rec A(-)) after mating with a Proteus mirabilis strain that carries the R-factor, R1. This plasmid, which represents a stable segregant of R1 that has lost all of the drug resistance determinants present on the parent R-factor but which has retained its ability to be transferred by conjugation, fulfills the functional definition of the R-factor transfer unit (RTF).

PubMed Disclaimer

References

    1. J Mol Biol. 1970 Jun 28;50(3):671-87 - PubMed
    1. Nature. 1969 Dec 27;224(5226):1273-7 - PubMed
    1. J Mol Biol. 1968 Sep 14;36(2):185-94 - PubMed
    1. J Bacteriol. 1961 May;81:669-78 - PubMed
    1. J Bacteriol. 1961 Aug;82:202-9 - PubMed

LinkOut - more resources