Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Oct;67(2):598-605.
doi: 10.1073/pnas.67.2.598.

A density label for membranes

C F Fox, J H Law, N Tsukagoshi, G Wilson

A density label for membranes

C F Fox et al. Proc Natl Acad Sci U S A. 1970 Oct.

Abstract

Bromostearic acid satisfies the essential fatty acid requirement of an unsaturated fatty acid auxotroph of Escherichia coli K12. Over 55% of the fatty acids derived from lipids isolated from membranes of cells grown in bromostearate-supplemented medium contained bromine, as established by gas chromatography and mass spectrometry of the methyl esters. During equilibrium centrifugation in sucrose gradients, the cytoplasmic membranes isolated from bromostearate-grown cells banded at a density approximately 0.06 g/cm(3) greater than membranes isolated from oleate- or elaidate-grown cells, affording a complete separation between membranes which contain brominated fatty acids and those which do not.

PubMed Disclaimer

References

    1. J Bacteriol. 1969 Nov;100(2):601-4 - PubMed
    1. J Bacteriol. 1967 Jan;93(1):427-37 - PubMed
    1. J Bacteriol. 1950 Jul;60(1):17-28 - PubMed
    1. Nature. 1951 Jul 28;168(4265):167 - PubMed
    1. J Bacteriol. 1970 Jul;103(1):273-4 - PubMed

LinkOut - more resources