Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Dec;68(12):3163-7.
doi: 10.1073/pnas.68.12.3163.

Hydrolysis of fMet-tRNA by peptidyl transferase

C T Caskey, A L Beaudet, E M Scolnick, M Rosman

Hydrolysis of fMet-tRNA by peptidyl transferase

C T Caskey et al. Proc Natl Acad Sci U S A. 1971 Dec.

Abstract

Escherichia coli and rabbit reticulocyte (f[(3)H]Met-tRNA.AUG.ribosome) intermediates undergo hydrolysis, with release of f[(3)H]methionine, upon addition of tRNA or CpCpA in the presence of acetone. This ribosomal catalyzed reaction has similar requirements, pH optimum, and antibiotic sensitivity to those of peptidyl transferase. Two antibiotics, lincomycin with E. coli ribosomes and anisomycin with reticulocyte ribosomes, inhibit peptide-bond formation and transesterification activities of peptidyl transferase, but stimulate hydrolysis of f[(3)H]Met-tRNA. Earlier studies have suggested peptidyl transferase activity is essential for R factor-dependent hydrolysis of f((3)H)Met-tRNA. These studies indicate that peptidyl transferase has the capacity for f((3)H)Met-tRNA hydrolysis and, therefore, may be responsible for peptidyl-tRNA cleavage during peptide chain termination.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1966 Oct 20;25(2):233-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1796-800 - PubMed
    1. J Mol Biol. 1967 Aug 28;28(1):161-5 - PubMed
    1. Eur J Biochem. 1968 Nov;6(2):309-16 - PubMed
    1. Nature. 1969 Apr 26;222(5191):356-8 - PubMed

MeSH terms

LinkOut - more resources