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. 1971 Sep;68(9):2145-8.
doi: 10.1073/pnas.68.9.2145.

Purification of the araC protein

G Wilcox, K J Clemetson, D V Santi, E Englesberg

Purification of the araC protein

G Wilcox et al. Proc Natl Acad Sci U S A. 1971 Sep.

Abstract

The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.

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References

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