Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Sep;68(9):2268-72.
doi: 10.1073/pnas.68.9.2268.

Purification and in vitro transcription of a transfer RNA gene

V Daniel, J S Beckmann, S Sarid, J I Grimberg, M Herzberg, U Z Littauer

Purification and in vitro transcription of a transfer RNA gene

V Daniel et al. Proc Natl Acad Sci U S A. 1971 Sep.

Abstract

A gene specifying tyrosine transfer RNA has been purified and transcribed in vitro. The purification procedure made use of two specialized transducing phages carrying the tRNA(Tyr) gene of Escherichia coli inserted into their DNA in opposite orientations. The separated heavy strands of the two phages were annealed and the single-stranded tails of the resulting hybrid were removed by digestion with Neurospora endonuclease. The size of the purified double-stranded structures was determined by electron microscopy. These isolated duplexes served as template for the in vitro transcription of tRNA(Tyr)-like molecules.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1968 Mar;59(3):792-9 - PubMed
    1. J Biol Chem. 1965 Mar;240:1287-93 - PubMed
    1. Biophys J. 1968 Oct;8(10):1104-18 - PubMed
    1. Can J Biochem. 1968 Oct;46(10):1285-91 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1968;33:839-42 - PubMed

LinkOut - more resources