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. 1965;6(4):318-28.
doi: 10.1186/BF03547089.

Quantitative determination of proteolytic, lipolytic and haemolytic activities in semen of some animal species

K Fossum, O Lyngset, J Aamdal

Quantitative determination of proteolytic, lipolytic and haemolytic activities in semen of some animal species

K Fossum et al. Acta Vet Scand. 1965.

Abstract
in English, German, Norwegian

1. Methods for quantitative determination of proteolytic, lipolytic and haemolytic activities and egg yolk factors in semen are described.

2. Comparative quantitative determinations of these enzyme activities in the semen of goat, ram, bull, boar, dog and horse have been carried out.

3. Special interest attaches to the egg yolk turbidity factor which is found in goat semen and, in small concentrations, also in boar semen.

4. As each species appears to have its specific enzyme pattern, regular enzymatic control does not seem to have great significance.

1. Methoden zur quantitativen Bestimmung der proteolytischen, lipolytischen und hämolytischen Aktivität sowie „egg yolk factors” in Samen werden beschrieben.

2. Vergleichende quantitative Bestimmungen der erwähnten Enzymaktivitäten in Samen von Ziegenbock, Widder, Stier, Eber, Hund und Hengst sind vorgenommen worden.

3. Besonderes Interesse ist mit dem „egg yolk turbidity factor” verbunden. Dieser kommt in Samen vom Ziegenbock und in kleineren Konzentrationen in Samen vom Eber vor.

4. Weil jede Tierart anscheinend ihr eigenes spezielles Enzymmuster besitzt, scheint eine routinemässige enzymatische Kontrolle von Samen nicht von grösserer Bedeutung zu sein.

1. Det er beskrevet metoder til kvantitativ bestemmelse av proteoly-tiske, lipolytiske og hemolytiske aktiviteter samt „egg yolk factors” i sæd.

2. Det er foretatt sammenlignende kvantitativ bestemmelse av de nevnte enzymaktiviteter i sæd av geitebukk, vær, okse, råne, hund og hingst.

3. Spesiell interesse knytter seg til “egg yolk turbidity factor”, som forekommer i sæd av geitebukk og i små konsentrasjoner også i sæd fra råne.

4. Da hver dyreart synes ha sitt spesielle enzymmønster synes rutinemessig enzymatisk kontroll av sæd ikke å være av vesentlig betydning.

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References

    1. Aamdal, J., O. Lyngset & K. Fossum: Toxic effect of lysolecithin on sperm. 1965. In press.
    1. Fossum K. Separation of haemolysin and egg yolk turbidity factor in cell-free extracts of Bacillus cereus. Acta path, microbiol. scand. 1963;59:400–406. - PubMed
    1. Iritani A, Nishikawa Y. Studies on the egg yolk coagulating factor (enzyme) in goat semen. III. Coagulating action to the fractionated yolk. Zootech, e Vet. 1962;17:322–325.
    1. Iritani A, Nishikawa Y. Studies on the egg yolk coagulating factor in goat semen. III, IV & V. Jap. J. Animal Rep. 1963;8:109–121.
    1. Mann, T.: Biochemistry of semen and of the male reproductive tract. London & New York 1964.

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