In vitro cytotoxic effects of lymphoid cells from rats with experimental autoimmune nephrosis
- PMID: 4958208
- PMCID: PMC1579167
In vitro cytotoxic effects of lymphoid cells from rats with experimental autoimmune nephrosis
Abstract
A nephrotic syndrome was induced in randomly bred Sprague-Dawley rats by repeated intraperitoneal injections of homologous kidney extract and Freund's complete adjuvant. Cell injury in vitro was measured by estimating the release of radioactivity from damaged target cells labelled with 14C-thymidine. Various conditions influencing the reproducibility of the isotope method were investigated.
Blood lymphoid cells, lymph node cells and spleen cells from these rats damaged primary monolayer cultures of rat kidney cells after 18 hr incubation. Lymphoid cells from liver sensitized rats were also cytotoxic to rat kidney cells, whereas lymphoid cells from rats injected with other rat tissue extracts (lung or spleen) or given only adjuvant did not react. Primary cultures of rat lung cells were sensitive while cells of a rat liver cell strain were resistant to the cytotoxic action of lymphoid cells from kidney sensitized rats. Sera from nephrotic rats did not damage rat kidney cells under the experimental conditions used here.
Similar articles
-
Studies on lymphocyte transformation in nephrotoxic serum nephritis of the rat.J Immunol. 1969 Aug;103(2):364-8. J Immunol. 1969. PMID: 4184825 No abstract available.
-
An in vitro demonstration of cellular sensitivity in experimental autoimmune nephrosis in rats.Proc Soc Exp Biol Med. 1968 Apr;127(4):1217-22. doi: 10.3181/00379727-127-32914. Proc Soc Exp Biol Med. 1968. PMID: 5655668 No abstract available.
-
Experimental autoimmune nephrosis. Pathogenetic considerations.Med Exp Int J Exp Med. 1968;18(1):4-12. Med Exp Int J Exp Med. 1968. PMID: 5755115 No abstract available.
-
Transfer of an autoimmune nephrosis in the rat by means of lymph node cells.J Exp Med. 1962 Feb 1;115(2):421-38. doi: 10.1084/jem.115.2.421. J Exp Med. 1962. PMID: 13906990 Free PMC article.
-
Nephrosis in the rat induced by rat kidney extracts.Ann N Y Acad Sci. 1965 Jun 30;124(1):323-8. doi: 10.1111/j.1749-6632.1965.tb18967.x. Ann N Y Acad Sci. 1965. PMID: 5215766 No abstract available.
Cited by
-
Autoimmune renal disease. Development of renal lesions and circulating antibodies in rats immunized with homologous kidney fractions.Immunology. 1967 Aug;13(2):167-77. Immunology. 1967. PMID: 4952103 Free PMC article.
-
Cellular immunity in glomerulonephritis.Am J Pathol. 1982 Nov;109(2):227-43. Am J Pathol. 1982. PMID: 6753596 Free PMC article. Review. No abstract available.
-
Antinuclear factor in NZB-NZW mice: incidence and in vitro effects.Clin Exp Immunol. 1968 Sep;3(7):657-63. Clin Exp Immunol. 1968. PMID: 4883067 Free PMC article.
-
Experimental myocarditis: in vitro and in vivo studies of lymphocytes sensitized to heart extracts and group A streptococci.Immunology. 1971 Feb;20(2):225-32. Immunology. 1971. PMID: 5548569 Free PMC article.
-
Cellular immunity in experimental glomerulonephritis of rats. I. Delayed hypersensitivity and lymphocyte stimulation studies with renal tubular antigens.Immunology. 1971 May;20(5):755-66. Immunology. 1971. PMID: 4278022 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources