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. 1967 Feb 17;155(3764):838-9.
doi: 10.1126/science.155.3764.838.

Sporulation mutations induced by heat in Bacillus subtilis

J Northrop, R A Slepecky

Sporulation mutations induced by heat in Bacillus subtilis

J Northrop et al. Science. .

Abstract

When spores of Bacillus subtilis strain Marburg are heated (90 degrees to 100 degrees C) in a vacuum for 9 to 12 hours and then plated, numerous mutants are obtained, and very few spores are killed. Disproportionately large numbers of these mutants exhibit abnormal sporulation.

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