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. 1968 Mar;95(3):867-75.
doi: 10.1128/jb.95.3.867-875.1968.

Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients

F H Cahn, M S Fox

Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients

F H Cahn et al. J Bacteriol. 1968 Mar.

Abstract

A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bacteria are the only bacteria capable of incorporating transforming deoxyribonucleic acid.

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