Fertility of deep frozen boar spermatozoa: influence of thawing diluents and of boars
- PMID: 4968
- PMCID: PMC8383986
- DOI: 10.1186/BF03547942
Fertility of deep frozen boar spermatozoa: influence of thawing diluents and of boars
Abstract
PIP: 2 insemination trials were conducted to assess the effect of different thawing diluents on deep frozen boar spermatozoa. The diluents used were boar seminal plasma, protein free seminal plasma, isotonic glucose solution, and OLEP (fructose, sodium pyruvate, CaC12, MgC12, KCL, NaC1, and NaHC03). The electrolyte levels and pH and osmotic pressure of the latter are similar to those of boar seminal plasma. 139 gilts were inseminated. The percentage of fertilized ova was similar for OLEP, protein free seminal plasma, and boar seminal plasma. However, thawing in isotonic glucose solution gave markedly poorer results. There were also differences in the fertility of spermatozoa from the different boars, though these were independent of the effects of the various thawing diluents. The possible effects of the thawing diluents are discussed. The need for methods of selecting boars with spermatozoa of good freezing quality is emphasized.
References
-
- Alanko M. A clinical and experimental study. Thesis. 1974. Fertilization and early development of ova in A.I.-gilts, with special reference to the role of tubal sperm concentration; p. 90.
-
- Bane A. Acrosomal abnormality associated with sterility in boar. Proc. IV Int. Congr. Anim. Reprod., The Hague. 1961;4:810–817.
-
- Bedford J M. Biol. Reprod. 1970. Sperm capacitation and fertilization in mammals; pp. 128–158. - PubMed
-
- Boehnke H J, Hahn B, Hillman K H, Treu H, Lorrmann W, Zoder H F. Zur Tiefgefrierung von Ebersperma. 3. Mitteilung: Weitere Labor- und Besamungsversuche unter Verwendung von TEST und TESNaK-Verdünner sowie verschiedenen Auftanmethoden. (3. Communication: Deepfreezing of boar semen; Laboratory experiments and further field trials using TEST and TESNaK-diluent and different thawing methods) Dtsch. tierärztl. Wschr. 1974;81:336–339. - PubMed
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