Insulin-anti-insulin precipitates in the presence of various dextrans

Abstract

Visible insulin—anti-insulin immunoprecipitates in agar gel media are often difficult to obtain. A new technique has been developed which allows a marked increase in the visibility and in the resolution of immunoprecipitin bands. These effects were observed with dextran solutions of various molecular weights, which were infused into agar gel on electrophoretic slides, after the electrophoretic seperation and the double diffusion steps had taken place.