The action of dilute aqueous NN-dimethylhydrazine on bacterial cell walls

Abstract

1. Walls of certain Gram-positive bacteria dissolved on incubation with dilute aqueous NN-dimethylhydrazine in the presence of air, by a reaction that probably involves free radicals. 2. Under the conditions described, the soluble products from the peptidoglycan were almost all non-diffusible. After brief incubation of walls of some organisms with reagent, part of the peptidoglycan component was obtained as a high-molecular-weight gel, the viscosity of which was rapidly decreased by incubation with lysozyme. 3. The extent to which peptidoglycan dissolved varied with different organisms, depending possibly on the extent of cross-linking, but the nature of the bonds that were destroyed has not been established. 4. Teichoic acids and polysaccharides were solubilized by this treatment and could be isolated in high overall yield. 5. The procedure is valuable in the examination of the distribution of heteropolymers in walls, and has been used to show that the polysaccharide present in walls of Lactobacillus arabinosus 17-5 is phosphorylated and may account for 20% of the total phosphate of the wall.