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. 1969 Nov;18(5):824-33.
doi: 10.1128/am.18.5.824-833.1969.

Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation

Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation

J C Hierholzer et al. Appl Microbiol. 1969 Nov.

Abstract

Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.

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References

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