Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid

Abstract

Based on protein, deoxyribonucleic acid (DNA), and phosphorus analyses, adeno-associated satellite virus type 4 has a DNA content of 26.5%, significantly larger than type 1 which contains 18.9% DNA. Type 4 DNA contains 58 to 62% guanine + cytosine, as calculated from thermal denaturation temperature and buoyant density measurements. Simian adenovirus SV15, which serves as a helper virus for type 4 satellite, contains 13.7% DNA with a guanine + cytosine content of 55 to 57%. The DNA extracted from type 4 satellite virus is a linear, double-stranded molecule, as shown by thermal denaturation temperature profile measurements and by electron microscopy. The contour length and sedimentation coefficient of type 4 DNA indicate a molecular weight of 3.0 x 10(6) daltons.