Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Jul;118(3):457-65.
doi: 10.1042/bj1180457.

Purification and properties of two extracellular beta-lactamases from Bacillus cereus 569-H

S Kuwabara

Purification and properties of two extracellular beta-lactamases from Bacillus cereus 569-H

S Kuwabara. Biochem J. 1970 Jul.

Abstract

1. When Bacillus cereus 569/H was grown in a casamino acid (casein-hydrolysate) medium containing zinc sulphate rapid production of extracellular beta-lactamase II preceded that of beta-lactamase I. 2. beta-Lactamase I was separated from beta-lactamase II by fractional precipitation with ammonium sulphate. 3. beta-Lactamase I was purified by a process involving chromatography on Celite and DEAE-cellulose and beta-lactamase II by chromatography on DEAE-cellulose after denaturation of beta-lactamase I by heat. Both enzymes were obtained in crystalline form. 4. beta-Lactamase II prepared in this way appeared to have a higher molecular weight than beta-lactamase I and required Zn(2+) as a cofactor for both cephalosporinase and penicillinase activities.

PubMed Disclaimer

References

    1. Biochem J. 1956 Mar;62(3):401-3 - PubMed
    1. Br J Exp Pathol. 1952 Dec;33(6):587-600 - PubMed
    1. J Bacteriol. 1968 May;95(5):1513-9 - PubMed
    1. C R Hebd Seances Acad Sci. 1953 Jul 20;237(3):276-8 - PubMed
    1. Chem Br. 1968 Sep;4(9):397-402 - PubMed