Microcloning and replica plating of mammalian cells

Abstract

A technique for cloning single mammalian cells has been developed by means of a microculture method (0.010 milliliters per culture). A technique of replica plating has also been developed for use with clones of single cells in microcultures; this technique permits the screening of desired properties of many clones simultaneously before the growth of stock cultures. With these techniques an investigator can with limited budget, space, and personnel perform research using tissue culture systems.