Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Oct;19(4):669-76.

Precipitating antibodies to DNA induced by heat-denatured DNA-albumin conjugates in the rabbit

N R Forsén, M Alspaugh, E V Barnett

Precipitating antibodies to DNA induced by heat-denatured DNA-albumin conjugates in the rabbit

N R Forsén et al. Immunology. 1970 Oct.

Abstract

(1) Antibodies reactive with purified DNA in the complement fixation system and induced by active immunization of rabbits with methylated bovine serum albumin—DNA aggregate (MBSA—DNA) have been previously described. The present study demonstrates their reactivity in the quantitative and double diffusion preciptin systems. In these systems they have shown preferential reactivity with heat denatured DNA and great cross-reactivity with denatured DNA from various sources.

(2) Methylated rabbit serum albumin—DNA aggregate (MRSA—DNA) is an effective immunizing antigen resulting in the production of antibodies similar to those produced by MBSA—DNA.

(3) The precipitating antibodies in this system are 19S immunoglobulins and appear to be identical to the complement fixing antibodies.

(4) The precipitin reaction is not significantly inhibited by mono-nucleotides.

PubMed Disclaimer

References

    1. J Immunol. 1966 Mar;96(3):464-71 - PubMed
    1. J Immunol. 1966 May;96(5):755-63 - PubMed
    1. J Immunol. 1966 May;96(5):764-71 - PubMed
    1. Immunology. 1966 Dec;11(6):547-56 - PubMed
    1. Adv Immunol. 1967;6:231-52 - PubMed

LinkOut - more resources