Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168
- PMID: 4991424
- PMCID: PMC376128
- DOI: 10.1128/JVI.6.3.340-343.1970
Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168
Abstract
A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to metC. The link-age relationship between xtl and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being argO 1 metA metC xtl.
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