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. 1971 Jan;105(1):376-83.
doi: 10.1128/jb.105.1.376-383.1971.

Purification of intact flagella from Escherichia coli and Bacillus subtilis

M L DePamphilis, J Adler

Purification of intact flagella from Escherichia coli and Bacillus subtilis

M L DePamphilis et al. J Bacteriol. 1971 Jan.

Abstract

A procedure is described for the purification of bacterial flagella in the form of a filament-hook-basal body complex (intact flagella) free from detectable cell wall, membrane, or cytoplasmic material. Spheroplasts produced with lysozyme and ethylenediaminetetraacetic acid were lysed with Triton X-100, and the flagella were purified by (NH(4))(2)SO(4) precipitation, differential centrifugation, and CsCl gradient centrifugation. As much as 40% of the flagella were recovered, and they contained about one basal body per 4 to 6 mum of flagella. The same procedure developed for Escherichia coli was also successful for purifying intact flagella from Bacillus subtilis.

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