doi: 10.3109/10520297509117039.
A rapid plastic embedding technique for preparation of three-micron thick sections of decalcified hard tissue
- PMID: 49947
- DOI: 10.3109/10520297509117039
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A rapid plastic embedding technique for preparation of three-micron thick sections of decalcified hard tissue
Stain Technol.
1975 Mar.
Abstract
A 24 hour start-to-finish method is described for the preparation of three-micron-thick sections of decalcified hard tissues. Following acetone dehydration, the tissue to be embedded is infiltrated under vacuum with a series of graded clearing solutions which approach the content of the final methyl methacrylate mixture. After overnight in a 35 C oven, the plastic is polymerized by four hours heating at 42 C. Three-micron-thick sections are then easily prepared by using a Jung microtome for high resolution histologic or detailed autoradiographic procedures.
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