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. 1971 Nov;22(5):837-41.
doi: 10.1128/am.22.5.837-841.1971.

Staphylococcal enterotoxin B: solid-phase radioimmunoassay

H M Johnson, J A Bukovic, P E Kauffman, J T Peeler

Staphylococcal enterotoxin B: solid-phase radioimmunoassay

H M Johnson et al. Appl Microbiol. 1971 Nov.

Abstract

An immunoassay employing (125)I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated polystyrene tubes. Unlabeled enterotoxin competed with (125)I-labeled enterotoxin for antibody-combining sites. The uptake of (125)I-labeled toxin reflected the concentration of unlabeled toxin present. The test is sensitive to 1 to 5 ng of purified and crude enterotoxin B per ml, and cross-reactions with heterologous enterotoxins did not interfere with the specificity. This test possesses the combination of sensitivity and objectivity absent in current methods for assaying enterotoxin and provides a model for investigating other enterotoxin serotypes.

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References

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