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. 1971 Aug;4(2):126-9.
doi: 10.1128/iai.4.2.126-129.1971.

Rapid detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion

C A Miller, A W Anderson

Rapid detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion

C A Miller et al. Infect Immun. 1971 Aug.

Abstract

An experimental system is described for the detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion. The method is shown to be rapid, specific, and quantitative. As little as 14 mouse LD(50) per 0.1 ml of type A toxin was detected within 2 hr. When applied to experimentally contaminated foods such as canned tuna, pumpkin, spinach, green beans, and sausage, the technique detected botulinum toxin rapidly and identified it as to type and quantity. A specific rabbit type A antitoxin was produced for this in vitro system since the equine antitoxin (Center for Disease Control) tested in this experiment was found to be unsuitable.

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