Isolation and characterization of two cell types of Coxiella burneti phase I

Abstract

Two morphologically distinct cell types of Coxiella burneti phase I have been separated on the basis of unique buoyant densities. When centrifuged to equilibrium in cesium chloride or density gradients of sucrose or Renografin, the cells band in two zones. Electron micrographs of ultrathin sections of the two cesium chloride-separated cell types indicate a considerable number of morphological differences. The lower-density cells are small, compact, and rodshaped and have very dense nucleoids. The cell type of highest density is larger, rounded, and more pleomorphic, and the nucleoid filaments are more dispersed. The two cell types are nearly identical in sedimentation rates, and both infect chick yolk sac cells and are lethal to chick embryos. They convert to a mixture of cell types when cultured separately. Treatment with Formalin induces all cells to band at the same position when centrifuged to equilibrium in cesium chloride. The cell type variance was found to be independent of the antigenic phase phenomenon of C. burneti.