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Comparative Study
. 1975 Jun 5;43(3):291-4.
doi: 10.1007/BF00499711.

Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material

J Burns
Comparative Study

Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material

J Burns. Histochemistry. .

Abstract

The PAP procedure was compared with the peroxidase labelled antibody sandwich method and was found to be at least 20 times more sensitive. Background staining was reduced by the addition of normal swine serum to all the immune sera or by pretreating sections with it at the beginning of either method. The PAP procedure could be effectively reduced to a period of 1 hour or less.

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References

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    1. J Histochem Cytochem. 1974 Feb;22(2):128-30 - PubMed
    1. J Histochem Cytochem. 1972 Oct;20(10):829-31 - PubMed
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