Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Jul;20(1):89-93.
doi: 10.1016/0039-128x(72)90120-1.

Estrogen-induced metabolism of estradiol-17 in the rat uterus: a possible mechanism for the termination of estrogen action

C R Lyttle, P H Jellinck

Estrogen-induced metabolism of estradiol-17 in the rat uterus: a possible mechanism for the termination of estrogen action

C R Lyttle et al. Steroids. 1972 Jul.

Abstract

PIP: Treating immature female rats with estradiol sc, 1 mcg in saline or .1 mcg in oil for 2 days, induced a peroxidase in the uterus which converted carbon-14 labeled estradiol into water soluble products in vitro. Uterine extracts prepared by Klebanoff's method were incubated for 1 hour at 38 degrees C with labeled estradiol, hydrogen peroxide, dichlorophenol, and bovine serum albumin in sodium ponsphate buffer. After extracting the medium with ether, the radioactivity in both phases was determined and the steroids in the ether phase were identified by thin layer chromatography. .1 mcg estradiol injected in saline did not affect either yield of estrone or water soluble metabolites. .1 mcg estradiol given in oil or 1 mcg in saline increased both the yield of soluble metabolites over fourfold and the percent conversion of estradiol to estrone sevenfold (oil) and twofold (saline). Thus estradiol is metabolized in the uterus; this metabolism may be a local control for duration of estrogen action.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

LinkOut - more resources