Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979;20(3):313-20.
doi: 10.1186/BF03546593.

Rapid method for screening of immunoglobulins in porcine fetuses, using rocket immunoelectrophoresis. Application of an interspecies reaction between human and porcine mu-chain

K Dalsgaard, E Overby, J J Metzger, A Basse

Rapid method for screening of immunoglobulins in porcine fetuses, using rocket immunoelectrophoresis. Application of an interspecies reaction between human and porcine mu-chain

K Dalsgaard et al. Acta Vet Scand. 1979.

Abstract
in English, Norwegian

A method for screening of immunoglobulin levels in porcine fetuses is presented. The method is based on rocket electrophoresis. This method is sensitive, but when used for assaying immunoglobulins it is only semiquantitative. It was shown that sera from 11 hysterectomy-derived colostrum-deprived neonatal pigs from a normal gilt contained no IgM or IgA, but small amounts of IgG. Sera from 9 piglets isolated in the same way from a gilt infected with porcine parvovirus contained all 3 antibody classes. IgM was detected by an interspecies reaction using anti human μ-chain immunoglobulin.

En metode til “screening” af immunglobulinniveau i grisefostre fremlægges. Metoden er baseret på raketelektroforese. Denne metode er følsom, men kun semikvantitativ ved bestemmelse af immunglobuliner. Det påvistes, at sera fra 11 grise, som blev taget ved hysterektomi, og som ikke fik kolostrum, indeholdt små mængder IgG, men ikke IgM eller IgA. Sera fra 9 tilsvarende grise, som blev taget fra en gylt der var inficeret med porcint parvovirus, indeholdt alle 3 antistofklasser. IgM blev påvist ved en interspecies reaktion under anvendelse af anti human μ-kæde Immunglobulin.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Binns R M. Bone marrow and lymphoid cell injection of pig foetus resulting in transplantation tolerance of immunity and immunoglobulin production. Nature (Lond.) 1967;214:179–180. doi: 10.1038/214179a0. - DOI - PubMed
    1. Bourne F J. IgA immunoglobulin from porcine serum. Biochim. biophys. Res. Commun. 1969;36:138–145. doi: 10.1016/0006-291X(69)90660-3. - DOI - PubMed
    1. Bourne F J. Gamma globulins in pre-colostral piglet serum. Res. vet. Sci. 1974;17:36–38. doi: 10.1016/S0034-5288(18)33704-4. - DOI - PubMed
    1. Bourne F J, Curtis J, Johnson R H, Collings D F. Antibody formation in porcine fetuses. Res. vet. Sci. 1974;16:223–227. doi: 10.1016/S0034-5288(18)33755-X. - DOI - PubMed
    1. Curtis J, Bourne F J. Immunoglobulin quantitation in sow serum, colostrum and milk and the serum of young pigs. Biochim. biophys. Acta (Amst.) 1971;236:319–332. doi: 10.1016/0005-2795(71)90181-4. - DOI - PubMed

Substances

LinkOut - more resources