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. 1979;20(3):329-42.
doi: 10.1186/BF03546595.

Binding of lipase, amylase and protease to intestinal epithelium as affected by carbohydrates and lectins in vitro

M Sandholm, M L Scott

Binding of lipase, amylase and protease to intestinal epithelium as affected by carbohydrates and lectins in vitro

M Sandholm et al. Acta Vet Scand. 1979.

Abstract
in English, Norwegian

Intraluminal digestive enzymes were shown to bind to chick intestinal epithelial surface (glycocalyx). Affinity of the intestinal epithelium for the enzymes decreased in the order, lipase > amylase > protease. The plant hemagglutinins, Con A, phytohemagglutinin, pokeweed mitogen and raw soybean effectively released lipase and amylase from the glycocalyx. Based on specific inhibition of binding by sugars, such as fucose and N-acetylated sugars, lipase and amylase appeared to be bound to blood group antigen-like sugar moieties on the glycocalyx of the microvilli.

Tarminnehållets pankreashydrolaser adsorberades på ut- och in-vända (inverterade) tarmsegmenter. När segmenterna eluerades i en serie provrör med buffrade koksaltlösningar, frigjordes den proteoly-tiska aktiviteten lättare än den amylolytiska eller lipolytiska aktiviteten. Rangordningen för affiniteten måste därför vara: lipas > amylas > proteas.

För att påvisa, att den epiteliala adsorptionen av pankreashydrolaser är en specifik process, gjordes försök att förhindra adsorptionen eller att frigöra den adsorberade enzymaktiviteten med olika sockerarter (som finns i den normala epiteliala kolhydratsubstansen, glycocalyx). Fucos och N-acetylerade sockerarter frigjorde lipas och amylas aktivitet. Växt hemagglutininer, som reagerar med sockerarter på cellytorna, frigjorde effektivt enzymaktivitetet.

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References

    1. Bennett G, Le Blond C P. Formation of cell coat material for the whole surface of columnar cells in the rat small intestine, as visualized by autoradiography with fucose-3H. J. Cell Biol. 1970;46:409–416. doi: 10.1083/jcb.46.2.409. - DOI - PMC - PubMed
    1. Brady P G, Vannier A M, Banwell J G. Identification of dietary lectin, wheat germ agglutinin, in human intestinal contents. Gastroenterology. 1978;75:236–239. doi: 10.1016/0016-5085(78)90409-2. - DOI - PubMed
    1. Cohen, E., ed.: Biomedical perspectives of agglutinins of invertebrate and plant origins. Ann, N. Y. Acad. Sci. 1974, 234, 1–412.
    1. Etzler M E. Use of plant agglutinins in characterization of glycoproteins and glycolipids from mammalian cells. Ann. N. Y. Acad. Sci. 1974;234:260–275. doi: 10.1111/j.1749-6632.1974.tb53038.x. - DOI - PubMed
    1. Forstner G C. Surface sugar in the intestine. Amer. J. med. Sci. 1969;258:172–180. doi: 10.1097/00000441-196909000-00004. - DOI - PubMed

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