doi: 10.1016/0005-2787(79)90078-9.
The maxi-circle of Trypanosoma brucei kinetoplast DNA
- PMID: 508755
- DOI: 10.1016/0005-2787(79)90078-9
Item in Clipboard
The maxi-circle of Trypanosoma brucei kinetoplast DNA
Biochim Biophys Acta.
.
Abstract
We have constructed a fragment map of the maxi-circle component from kinetoplast DNA networks of Trypanosoma brucei brucei (East African Trypanosomiasis Research Organization strain No. 427), using restriction endonucleases PstI, HapII, EcoRI, BglI, HindIII, HhaI, SstI, XbaI, HaeIII and Sau-96I. Although the 20 kilo-base pair map contains 30 fragments, there is a 6.5 kilo-base pair 'silent' segment which lacks recognition sites for any of these enzymes. In CsCl intact kinetoplast DNA has a buoyant density of 1.690 g/cm3, whereas a kinetoplast DNA fraction enriched in maxi-circle sequences contains an additional component at 1.682 g/cm3. The possibility that the 'silent' segment is very rich in A + T is discussed.
Similar articles
-
Isolation and characterization of kinetoplast DNA from bloodstream form of Trypanosoma brucei.J Cell Biol. 1978 Feb;76(2):293-309. doi: 10.1083/jcb.76.2.293. J Cell Biol. 1978. PMID: 10605439 Free PMC article.
-
Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains.Biochim Biophys Acta. 1980 Dec 11;610(2):197-210. doi: 10.1016/0005-2787(80)90001-5. Biochim Biophys Acta. 1980. PMID: 6260147
-
Further analysis of intraspecific variation in Trypanosoma brucei using restriction site polymorphisms in the maxi-circle of kinetoplast DNA.Mol Biochem Parasitol. 1985 Apr;15(1):21-36. doi: 10.1016/0166-6851(85)90026-x. Mol Biochem Parasitol. 1985. PMID: 2985985
-
Quantitation of genetic differences between Trypanosoma brucei gambiense, rhodesiense and brucei by restriction enzyme analysis of kinetoplast DNA.Mol Biochem Parasitol. 1981 Jun;3(2):117-31. doi: 10.1016/0166-6851(81)90011-6. Mol Biochem Parasitol. 1981. PMID: 6166860
-
[The mitochondrial genome of the trypanosomatid kinetoplast: gene organization and transcription of the maxicircle DNA].Tsitologiia. 1993;35(3):4-23. Tsitologiia. 1993. PMID: 8391174 Review. Russian.
Cited by
-
Characterization of the DNA duplication-transposition that controls the expression of two genes for variant surface glycoproteins in Trypanosoma brucei.Nucleic Acids Res. 1982 Jan 22;10(2):593-609. doi: 10.1093/nar/10.2.593. Nucleic Acids Res. 1982. PMID: 6278423 Free PMC article.
-
The genomic basis of host and vector specificity in non-pathogenic trypanosomatids.Biol Open. 2022 Apr 15;11(4):bio059237. doi: 10.1242/bio.059237. Epub 2022 May 3. Biol Open. 2022. PMID: 35373253 Free PMC article.
-
An analysis of cosmid clones of nuclear DNA from Trypanosoma brucei shows that the genes for variant surface glycoproteins are clustered in the genome.Nucleic Acids Res. 1982 Oct 11;10(19):5905-23. doi: 10.1093/nar/10.19.5905. Nucleic Acids Res. 1982. PMID: 6292859 Free PMC article.
-
Novel pattern of editing regions in mitochondrial transcripts of the cryptobiid Trypanoplasma borreli.EMBO J. 1994 Nov 1;13(21):5086-98. doi: 10.1002/j.1460-2075.1994.tb06838.x. EMBO J. 1994. PMID: 7525275 Free PMC article.
-
Characterization of the expression-linked gene copies of variant surface glycoprotein 118 in two independently isolated clones of Trypanosoma brucei.Nucleic Acids Res. 1982 Apr 10;10(7):2353-66. doi: 10.1093/nar/10.7.2353. Nucleic Acids Res. 1982. PMID: 6283477 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
