Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate
- PMID: 508762
- DOI: 10.1016/0005-2787(79)90097-2
Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate
Abstract
The very useful radiolabeled ATP analog, adenosine 5-O-(3-[35S] thiotriphosphate) or [35S] ATPgammaS, has been prepared by a technique based on the thiophosphorylation of ADP that results in much higher yields of [35S] ATPgammaS than does a thiophosphate exchange method [1].
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