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. 1971 Sep;107(3):840-5.
doi: 10.1128/jb.107.3.840-845.1971.

NH 2 -terminal residues of Neurospora crassa proteins

NH 2 -terminal residues of Neurospora crassa proteins

H M Rho et al. J Bacteriol. 1971 Sep.

Abstract

The NH(2)-terminal amino acid composition of the soluble and ribosomal proteins from Neurospora crassa mycelia and conidia was determined by the dinitrophenyl method. A nonrandom distribution of NH(2)-terminal amino acids was observed in the complex protein mixtures. Glycine, alanine, and serine accounted for 75% of the NH(2)-terminal amino acids, and glycine appeared most frequently in mature proteins of mycelia. The appearance of phenylalanine as one of the major NH(2)-termini in crude conidial fraction suggests that the composition of proteins may vary in different developmental stages.

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