Observations on cell volume, ultrastructure, mitochondrial conformation and vital-dye uptake in Ehrlich ascites tumor cells. Effects of inhibiting energy production and function of the plasma membrane

Abstract

The ultrastructure, volume, vital-dye uptake, ATP levels and amounts of intracelular water have been studied in Ehrlich ascites cells treated with two classes of inhibitors. Cell membrane injury with 10(-3) M parachloromercuribenzene sulfonate (PCMBS) resulted in a rapid increase in mean cell volume to 3 times the mean zero time value. Inhibition of respiration plus glycolysis with 10(-4) M iodoacetate (IAA) plus 10(-4) M antimycin resulted first in a slight decrease in volume and then later a slow increase. When glucose was present in the medium, this slow increase was very slight. Intracellular water determination followed closely the volume changes. Cell populations with decreased mean volumes showed dilatations of the ER and swelling of mitochondria, indicating that expansion of some intracellular compartments can occur at the expense of others. Vital-dye uptake in metabolic injury correlated to some extent with the presence of substrate, indicating that an effect of glucose on membrane permeability might be independent of its effects on ATP levels. Mitochondrial contractions appeared with PCMBS as early as 5 minutes, with or without glucose present. With glucose present, treatment with IAA and antimycin also resulted in similar contractions as well as ring-form contractions even at 5 minutes. Without glucose, IAA plus antimycin mainly produced the usual mitochondrial contractions and these developed much later in the course of the incubation, shortly before high-amplitude swelling. The relationship of these mitochondrial conformational changes to ADP/ATP ratios and ADP levels is discussed.