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. 1979 Nov 15;35(11):1505-6.
doi: 10.1007/BF01962812.

Effects of low temperatures on survival of frozen-thawed mouse embryos

H Miyamoto, T Ishibashi

Effects of low temperatures on survival of frozen-thawed mouse embryos

H Miyamoto et al. Experientia. .

Abstract

Almost no damage to mouse morulae was observed between 0 and -40 degrees C, and freezing damage to embryos in DMSO, glycerol or ethylene glycol occurred after exposure to -60, -50 or -50 degrees C, respectively. Cooling embryos in DMSO or glycerol to temperatures below -50 to -60 degrees C increased freezing damage. To the contrary, in the presence of ethylene glycol, no more damage occurred after exposure to temperatures below -50 degrees C.

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