The detection of antibody and demonstration of immunoglobulin class, with inert particles and indicator red cells

Abstract

Sephadex will adsorb protein non-covalently and this can be detected by mixed agglutination reactions with antibody and antigen coated red cells. Rheumatoid factor will adsorb to Sephadex and can be demonstrated by immunoglobulin coated red cells. BioGel particles show considerably less non-specific adsorption of protein and this makes them more suitable for the demonstration of antibody on antigen coated particles by means of antiglobulin reagents. Using a two step antiglobulin reaction it was possible to detect IgG, IgM and IgD anti-insulin antibody.