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. 1971 Mar;122(1):89-92.
doi: 10.1042/bj1220089.

An improved procedure for the isolation of 3-phosphoglycerate kinase from yeast

R K Scopes

An improved procedure for the isolation of 3-phosphoglycerate kinase from yeast

R K Scopes. Biochem J. 1971 Mar.

Abstract

3-Phosphoglycerate kinase has been isolated from yeast by a new procedure. Over 1g was obtained from 450g of granulated baker's yeast; it had a specific activity of up to 940units/mg at 30 degrees C. Six distinct crystalline forms have been grown, at least one of these being suitable for X-ray diffraction studies. The crystalline preparation is pure, judged by starch-gel or sodium dodecyl sulphate-polyacrylamide-gel electrophoresis; the latter method indicating that the enzyme is monomeric, with a molecular weight near to 50000.

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