A survey of the interaction of calcium ions with mitochondria from different tissues and species

Abstract

A survey was made of the capacity of mitochondria isolated from a number of different tissues and species to accumulate Ca(2+) from the suspending medium during electron transport. The species examined included the rat, mouse, rabbit, hamster, guinea pig, cow, chicken, turtle, blowfly, yeast and Neurospora crassa. The tissues examined included vertebrate liver, kidney, brain, heart, spleen, thyroid and adrenal cortex, and the flight muscle of the blowfly. The mitochondria from all vertebrate tissues examined showed: (a) stimulation of State 4 respiration by added Ca(2+) (Ca(2+)/~ activation ratio about 2.0), accompanied by accumulation of Ca(2+) and ejection of H(+), with a H(+)/Ca(2+) ratio about 1.0; (b) a requirement of phosphate for accumulation of large amounts of Ca(2+); (c) respiration-independent high-affinity binding sites for Ca(2+); (d) endogenous Ca(2+), which is largely released by uncoupling agents. However, mitochondria from yeast and blowfly flight muscle are unable to accumulate Ca(2+) in a respiration-dependent process and possess no high-affinity Ca(2+)-binding sites. These findings support the view that the high-affinity sites represent the ligand-binding sites of a specific Ca(2+) ;permease' or transport system in the membrane. The relatively high affinity for Ca(2+), which equals or exceeds the affinity for ADP, and the generally uniform characteristics of Ca(2+) transport in all the vertebrate mitochondria tested strongly suggest that respiration-linked Ca(2+) accumulation plays a general and fundamental role in vertebrate cell physiology.