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. 1971;12(3):393-401.
doi: 10.1186/BF03547738.

Diurnal and seasonal variations of serum enzyme activity in cattle and sheep

S Tollersrud, T W Gedde-Dahl

Diurnal and seasonal variations of serum enzyme activity in cattle and sheep

S Tollersrud et al. Acta Vet Scand. 1971.

Abstract
in English, German

In order to test the variation of enzyme activity in serum of cattle and sheep during the day, blood samples were taken at three hrs. interval from 6 a.m. to 9 p.m. The following enzymes were assayed: Aspartate aminotransferase (AspAT = GOT), alanine aminotransferase (AlAT = GPT), total lactate dehydrogenase (LDH), and a-hydroxybutyrate dehydrogenase (HBD). The variation between animals contributed by far to the greatest part of the total variation in clinical healthy animals. The time-of-day-dependant variation was less than 3 %, except for alanine aminotransferase.

During the first two weeks of spring pasture serum aspartate and alanine aminotransferase levels were significantly raised in both cows and ewes, compared with serum levels of the same animals on indoor feeding. No such increase occurred in total lactate dehydrogenase.

For å undersøke i hvilken grad aktiviteten av visse diagnostisk betydningsfulle enzymer i serum varierer i løpet av dagen, ble blodprøver tatt av seks storfe og seks sauer med tre timers mellomrom fra kl. 6 til kl. 21. Følgende enzymer ble undersøkt: Aspartataminotransferase (AspAT = GOT), alaninaminotransferase (A1AT = GPT), total laktatdehydrogenase (LDH) og a-hydroxybutyratdehydrogenase (HBD).

Forskjellen mellom dyr bidro med langt den største andelen av den totale variasjon. Den delen som kunne tilskrives tidspunktet på dagen, utgjorde mindre enn 3 % for alle enzymer, unntatt for alaninaminotransf erase.

Ved overgang fra inneforing til beite om våren, ble det funnet en signifikant stigning i begge de undersøkte transferaser både hos kyr og søyer, mens det ikke var noen forandring av LDH. Lam viste et noe annet bilde.

Variasjonene i løpet av dagen, og forandringene ved overgang fra inneforing til beite, vil sjelden spille noen praktisk rolle for vurderingen av enzymverdier i den kliniske diagnostikk. For mer vitenskapelige undersøkelser på dette felt bør en imidlertid nøye presisere under hvilke betingelser forsøkene er utført, for å kunne sammenlikne sine resultater med andres.

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References

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    1. Tollersrud S. Stability of some serum enzymes in sheep, cattle, and swine during storage at different temperatures. Acta vet. scand. 1969;10:359–371. - PMC - PubMed

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