Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Nov;125(1):303-8.
doi: 10.1042/bj1250303.

Digestion of an exogenous protein by rat yolk-sac cultured in vitro

K E Williams, J B Lloyd, M Davies, F Beck

Digestion of an exogenous protein by rat yolk-sac cultured in vitro

K E Williams et al. Biochem J. 1971 Nov.

Abstract

Yolk-sacs were removed from 17.5-day pregnant rats injected 2-5h previously with (125)I-labelled bovine serum albumin. The specific activities of acid phosphatase and acid proteinase, and the specific radioactivities (trichloroacetic acid-insoluble and trichloroacetic acid-soluble) were measured in subcellular fractions prepared by homogenization and differential centrifugation. The conversion of acid-insoluble into acid-soluble radioactivity within cultured tissue was followed and the nature of the liberated products was investigated by gel chromatography. The results are consistent with the protein entering lysosomes and being digested there. The radiolabel was released chiefly as free iodotyrosine.

PubMed Disclaimer

References

    1. Biochem J. 1964 Jul;92(1):217-24 - PubMed
    1. Teratology. 1969 Nov;2(4):283-96 - PubMed
    1. Anat Rec. 1959 Feb;133(2):115-28 - PubMed
    1. Anal Biochem. 1963 Sep;6:223-33 - PubMed
    1. Anal Biochem. 1965 Aug;12:290-5 - PubMed