Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 May;122(4):575-82.
doi: 10.1042/bj1220575.

Unsuitability of indoxyl acetate as a substrate for the assay of testicular hyaluronidase

C Rhodes, K S Dodgson, A H Olavesen, B Högberg

Unsuitability of indoxyl acetate as a substrate for the assay of testicular hyaluronidase

C Rhodes et al. Biochem J. 1971 May.

Abstract

The most recently published method for the assay of testicular hyaluronidase preparations was based on the premise that the enzyme also exhibited carboxylesterase activity towards indoxyl acetate. Studies on the relative enzyme activities of various hyaluronidase preparations towards hyaluronate and indoxyl acetate, the relative stabilities towards pH, temperature and mechanical shaking and the behaviour towards a variety of inhibitors, showed that the activities towards the two substrates reflected the presence of at least two different enzyme systems in the preparations. Gel chromatography and polyacrylamide-gel-electrophoresis experiments confirmed these conclusions and the collective findings clearly establish that methods based on the use of indoxyl acetate cannot be employed to measure testicular hyaluronidase activity.

PubMed Disclaimer

References

    1. J Biol Chem. 1967 Feb 10;242(3):437-40 - PubMed
    1. Bull World Health Organ. 1957;16(2):291-4 - PubMed
    1. J Biol Chem. 1951 Feb;188(2):485-90 - PubMed
    1. Biochem J. 1965 Nov;97(2):380-8 - PubMed
    1. J Biol Chem. 1963 Nov;238:3522-7 - PubMed