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. 1975 Aug;356(8):1313-6.
doi: 10.1515/bchm2.1975.356.2.1313.

Relevance of three-dimensional reconstructions of stain distributions for structural analysis of biomolecules

W Baumeister, M Hahn

Relevance of three-dimensional reconstructions of stain distributions for structural analysis of biomolecules

W Baumeister et al. Hoppe Seylers Z Physiol Chem. 1975 Aug.

Abstract

The relevance of a three-dimensional model of a protein molecule is discussed in the context of artefacts which inevitably occur during the preparation and imaging process. It is emphasized that even under optimistic assumptions, there-construction of a structure complementary to a given spatial stain distribution cannot, at resolutions below 10-15 A, be regarded as being identical with the real protein structure.

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