doi: 10.1007/BF00495033.
The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts
- PMID: 521318
- DOI: 10.1007/BF00495033
Item in Clipboard
The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts
Histochemistry.
1979.
Abstract
Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm.