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. 1979 Sep;54(5):257-60.
doi: 10.3109/10520297909110681.

Micro-buffy coats of whole blood: a method for the electron microscopic study of mononuclear cells

J F Nunes, J O Soares, A P Alves de Matos

Micro-buffy coats of whole blood: a method for the electron microscopic study of mononuclear cells

J F Nunes et al. Stain Technol. 1979 Sep.

Abstract

A method for the electron microscopic study of human peripheral lymphocytes by which very small buffy coats are obtained through centrifugation of heparinized whole blood in glass or plastic microhematocrit tubes is presented. This method is time saving and efficient, yielding well preserved material and a comparatively large number of mononuclear cells (mainly lymphocytes) in each thin section.

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