doi: 10.1073/pnas.62.3.644.
23Na nuclear magnetic resonance relaxation studies of sodium ion interaction with soluble RNA
- PMID: 5256995
- PMCID: PMC223645
- DOI: 10.1073/pnas.62.3.644
Item in Clipboard
23Na nuclear magnetic resonance relaxation studies of sodium ion interaction with soluble RNA
Proc Natl Acad Sci U S A.
1969 Mar.
Abstract
Interactions between (23)Na(+) and soluble RNA in aqueous solution are studied with the use of (23)Na nuclear magnetic resonance. At low concentrations of NaCl, the interactions obey a simple equilibrium model with a formation constant log (K(f))(3) = 2.8 +/- 0.3. The relaxation rate of the bound sodium is found to be T(1B) (-1) = 222 +/- 19 sec(-1) compared to that of free sodium T(1F) (-1) = 17.5 sec(-1). At high NaCl concentrations, the system deviates from the model, possibly owing to aggregation of the soluble RNA.
Similar articles
-
Nuclear magnetic resonance of 23Na ions interacting with the gramicidin channel.Biophys J. 1985 Oct;48(4):643-62. doi: 10.1016/S0006-3495(85)83820-0. Biophys J. 1985. PMID: 2413919 Free PMC article.
-
Spin-echo nuclear magnetic resonance evidence for complexing of sodium ions in muscle, brain, and kidney.Biophys J. 1970 Sep;10(9):843-58. doi: 10.1016/S0006-3495(70)86339-1. Biophys J. 1970. PMID: 5496905 Free PMC article.
-
Importance of coulombic end effects on cation accumulation near oligoelectrolyte B-DNA: a demonstration using 23Na NMR.Biophys J. 1995 Mar;68(3):1063-72. doi: 10.1016/S0006-3495(95)80281-X. Biophys J. 1995. PMID: 7756526 Free PMC article.
-
A review of 23Na nuclear magnetic resonance spectroscopy for the in vitro study of cellular sodium metabolism.Scand J Clin Lab Invest. 1990 Sep;50(5):497-507. doi: 10.1080/00365519009089164. Scand J Clin Lab Invest. 1990. PMID: 2237262 Review.
-
23Na NMR relaxation studies of the Na-DNA/drug interaction.Chemphyschem. 2001 Oct 15;2(10):569-75. doi: 10.1002/1439-7641(20011015)2:10<569::AID-CPHC569>3.0.CO;2-S. Chemphyschem. 2001. PMID: 23686872 Review.
Cited by
-
Rate theory calculation of gramicidin single-channel currents using NMR-derived rate constants.Proc Natl Acad Sci U S A. 1980 Apr;77(4):2028-32. doi: 10.1073/pnas.77.4.2028. Proc Natl Acad Sci U S A. 1980. PMID: 6154942 Free PMC article.
-
Potassium-39 NMR of K+ interaction with the gramicidin channel and NMR-derived conductance ratios for Na+, K+ and Rb+.J Membr Biol. 1986;89(1):107-11. doi: 10.1007/BF01870900. J Membr Biol. 1986. PMID: 2420992
-
Nuclear magnetic resonance studies on intracellular sodium in human erythrocytes and frog muscle.Biophys J. 1973 Jan;13(1):56-71. doi: 10.1016/S0006-3495(73)85969-7. Biophys J. 1973. PMID: 4541139 Free PMC article.
-
The Alkali Ion-DNA Interaction as Reflected in the Nuclear Relaxation Rates of Na and Rb.Proc Natl Acad Sci U S A. 1975 Jan;72(1):245-7. doi: 10.1073/pnas.72.1.245. Proc Natl Acad Sci U S A. 1975. PMID: 16592212 Free PMC article.
-
Relative binding affinities of monovalent cations for double-stranded DNA.Proc Natl Acad Sci U S A. 1980 Jun;77(6):3085-9. doi: 10.1073/pnas.77.6.3085. Proc Natl Acad Sci U S A. 1980. PMID: 16592827 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
