Disk diffusion testing of susceptibility of Mycobacterium fortuitum and Mycobacterium chelonei to antibacterial agents
- PMID: 526002
- PMCID: PMC352914
- DOI: 10.1128/AAC.16.5.611
Disk diffusion testing of susceptibility of Mycobacterium fortuitum and Mycobacterium chelonei to antibacterial agents
Abstract
Although recent studies have suggested that some antibacterial agents have good activity against the rapidly growing mycobacteria Mycobacterium fortuitum and Mycobacterium chelonei, an easily applicable method for susceptibility testing of clinical isolates is not yet available. We evaluated a disk diffusion method with Mueller-Hinton agar and 48-h readings with 59 strains of M. fortuitum and 11 strains of M. chelonei and compared the results to agar dilution susceptibilities for nine antimicrobial agents. All isolates were susceptible to 16 micrograms of amikacin or kanamycin per ml with minimum zone diameters of 14 and 18 mm, respectively. Amikacin inhibited 100% of isolates of M. fortuitum at 2 micrograms/ml, whereas 10 of 11 (91%) of M. chelonei strains had minimum inhibitory concentrations of 4.0 micrograms/ml or greater. Doxycycline and minocycline had almost identical activities, inhibiting 44% of strains at 4.0 micrograms/ml, and both allowed easy differentiation between susceptible and resistant strains by disk diffusion. Although most isolates of M. chelonei grew better on 7H10 agar, this media gave two- to eight-fold higher minimum inhibitory concentrations than were obtained with Mueller-Hinton agar. Disk diffusion susceptibility testing appears to be a simple and reliable means of predicting susceptibility results for M. fortuitum and most isolates of M. chelonei by the agar dilution method.
Similar articles
-
Agar disk elution method for susceptibility testing of Mycobacterium marinum and Mycobacterium fortuitum complex to sulfonamides and antibiotics.Antimicrob Agents Chemother. 1983 Oct;24(4):486-93. doi: 10.1128/AAC.24.4.486. Antimicrob Agents Chemother. 1983. PMID: 6651277 Free PMC article.
-
Antimicrobial susceptibility testing of Mycobacterium fortuitum complex.Antimicrob Agents Chemother. 1979 Jun;15(6):754-7. doi: 10.1128/AAC.15.6.754. Antimicrob Agents Chemother. 1979. PMID: 475359 Free PMC article.
-
Rapidly growing mycobacteria: testing of susceptibility to 34 antimicrobial agents by broth microdilution.Antimicrob Agents Chemother. 1982 Aug;22(2):186-92. doi: 10.1128/AAC.22.2.186. Antimicrob Agents Chemother. 1982. PMID: 6927280 Free PMC article.
-
The rapidly growing mycobacteria--Mycobacterium fortuitum and Mycobacterium chelonei.Infect Control. 1985 Jul;6(7):283-8. doi: 10.1017/s0195941700061762. Infect Control. 1985. PMID: 3891655 Review.
-
In vitro evaluations of amikacin: an assessment of the currently used methods of disk diffusion and dilution susceptibility, antimicrobial synergy, and the measurement of amikacin concentrations.Am J Med. 1986 Jun 30;80(6B):88-97. doi: 10.1016/0002-9343(86)90485-7. Am J Med. 1986. PMID: 3524220 Review.
Cited by
-
Agar disk elution method for susceptibility testing of Mycobacterium marinum and Mycobacterium fortuitum complex to sulfonamides and antibiotics.Antimicrob Agents Chemother. 1983 Oct;24(4):486-93. doi: 10.1128/AAC.24.4.486. Antimicrob Agents Chemother. 1983. PMID: 6651277 Free PMC article.
-
Real time PCR for the rapid identification and drug susceptibility of Mycobacteria present in Bronchial washings.BMC Infect Dis. 2016 Oct 26;16(1):607. doi: 10.1186/s12879-016-1943-y. BMC Infect Dis. 2016. PMID: 27782812 Free PMC article.
-
Mycobacterium chelonae abscesses associated with biomesotherapy, Australia, 2008.Emerg Infect Dis. 2013;19(9):1493-5. doi: 10.3201/eid1909.120898. Emerg Infect Dis. 2013. PMID: 23968779 Free PMC article.
-
Evaluation of Etest for rapid susceptibility testing of Mycobacterium chelonae and M. fortuitum.J Clin Microbiol. 1994 Aug;32(8):1846-9. doi: 10.1128/jcm.32.8.1846-1849.1994. J Clin Microbiol. 1994. PMID: 7989530 Free PMC article.
-
A synergistic investigation of azo-thiazole derivatives incorporating thiazole moieties: a comprehensive exploration of their synthesis, characterization, computational insights, solvatochromism, and multimodal biological activity assessment.RSC Adv. 2023 Nov 24;13(49):34534-34555. doi: 10.1039/d3ra06469g. eCollection 2023 Nov 22. RSC Adv. 2023. PMID: 38024963 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources