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. 1969 Aug;63(4):1431-8.
doi: 10.1073/pnas.63.4.1431.

Deacylated tRNA-phe binding to a reticulocyte ribosomal site for the initiation of polyphenylalanine synthesis

W J Culp, W L McKeehan, B Hardesty

Deacylated tRNA-phe binding to a reticulocyte ribosomal site for the initiation of polyphenylalanine synthesis

W J Culp et al. Proc Natl Acad Sci U S A. 1969 Aug.

Abstract

The initiation of polyphenylalanine synthesis at low MgCl(2) concentration in the reticulocyte transfer system has been found to have a stringent requirement for deacylated tRNA(Phe). An initial poly-U-directed complex between deacylated tRNA(Phe) and ribosomes is formed. The onset of polyphenylalanine synthesis causes the rapid release of tRNA(Phe) from the complex. Thermal dissociation studies indicate that deacylated tRNA(Phe) and phenylalanyl-tRNA are bound to the same ribosomes. A total of three ribosomal tRNA binding sites is indicated.

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References

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