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. 1970 Jan;65(1):129-36.
doi: 10.1073/pnas.65.1.129.

Isolation of microtubule protein from cultured mouse neuroblastoma cells

J B Olmsted, K Carlson, R Klebe, F Ruddle, J Rosenbaum

Isolation of microtubule protein from cultured mouse neuroblastoma cells

J B Olmsted et al. Proc Natl Acad Sci U S A. 1970 Jan.

Abstract

The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.

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References

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