doi: 10.1073/pnas.65.1.129.
Isolation of microtubule protein from cultured mouse neuroblastoma cells
- PMID: 5263744
- PMCID: PMC286201
- DOI: 10.1073/pnas.65.1.129
Item in Clipboard
Isolation of microtubule protein from cultured mouse neuroblastoma cells
Proc Natl Acad Sci U S A.
1970 Jan.
Abstract
The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.
Similar articles
-
Precipitation of proteins by vinblastine and calcium ions.Proc Natl Acad Sci U S A. 1970 Jul;66(3):807-14. doi: 10.1073/pnas.66.3.807. Proc Natl Acad Sci U S A. 1970. PMID: 5269244 Free PMC article.
-
Vinblastine-induced precipitation of microtubule protein.Science. 1969 Aug 1;165(3892):498-9. doi: 10.1126/science.165.3892.498. Science. 1969. PMID: 5815703
-
Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella.Proc Natl Acad Sci U S A. 1971 Sep;68(9):2273-7. doi: 10.1073/pnas.68.9.2273. Proc Natl Acad Sci U S A. 1971. PMID: 5289385 Free PMC article.
-
An investigation of the microtubule protein in the mature oocytes of Urechis caupo.Exp Cell Res. 1973 Oct;81(2):342-50. doi: 10.1016/0014-4827(73)90524-7. Exp Cell Res. 1973. PMID: 4796635 No abstract available.
-
[Microtubules and morphopoietic mechanism].Annee Biol. 1970 Jan-Feb;9(1):1-76. Annee Biol. 1970. PMID: 4984935 Review. French. No abstract available.
Cited by
-
Screening of tau protein kinase inhibitors in a tauopathy-relevant cell-based model of tau hyperphosphorylation and oligomerization.PLoS One. 2020 Jul 21;15(7):e0224952. doi: 10.1371/journal.pone.0224952. eCollection 2020. PLoS One. 2020. PMID: 32692785 Free PMC article.
-
Tubulin determination by an isotope dilution-vinblastine precipitation method. The tubulin content of Spisula eggs and embryos.J Cell Biol. 1973 Dec;59(3):755-62. doi: 10.1083/jcb.59.3.755. J Cell Biol. 1973. PMID: 4586678 Free PMC article. No abstract available.
-
Fine structure and innervation of the avian adrenal glands. II. Cholinergic innervation of adrenal chromaffin cells.Z Zellforsch Mikrosk Anat. 1973 Dec 6;145(3):417-42. doi: 10.1007/BF00307165. Z Zellforsch Mikrosk Anat. 1973. PMID: 4772596 No abstract available.
-
Microtubule protein. Identification in and transport to nerve endings.J Cell Biol. 1971 Oct;51(1):138-47. doi: 10.1083/jcb.51.1.138. J Cell Biol. 1971. PMID: 5111874 Free PMC article.
-
Condensing vacuole conversion and zymogen granule discharge in pancreatic exocrine cells: metabolic studies.J Cell Biol. 1971 Mar;48(3):503-22. doi: 10.1083/jcb.48.3.503. J Cell Biol. 1971. PMID: 5547590 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
