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. 1969 Nov;64(3):1065-71.
doi: 10.1073/pnas.64.3.1065.

Pre-fork synthesis: a model for DNA replication

E H Haskell, C I Daverin

Pre-fork synthesis: a model for DNA replication

E H Haskell et al. Proc Natl Acad Sci U S A. 1969 Nov.

Abstract

A model of DNA replication is presented in which DNA synthesis is continuously initiated from parental strand nicks and occurs, with conservation of helix winding number, ahead of the so-called replicating fork. The fork in this model is the locus of unwinding of already replicated, but presumably unstable, DNA. The model, involving Okazaki's notion of multiple initiation, is based upon the properties of Kornberg's DNA polymerase and accounts for the presence of single-stranded nascent DNA fragments in cell lysates. In addition to acting as sites of initiation, the parental strand nicks are implicated as sites of free rotation allowing unwinding of the replicated DNA.

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References

    1. J Mol Biol. 1965 Feb;11:373-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1451-5 - PubMed
    1. J Mol Biol. 1966 May;17(1):1-9 - PubMed
    1. J Biol Chem. 1967 Mar 10;242(5):936-50 - PubMed
    1. Biochim Biophys Acta. 1966 Dec 21;129(3):651-4 - PubMed

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