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. 1979 Mar;63(180):69-82.

[Growth and morphologic characteristics of normal human endometrial cells in primary culture]

[Article in French]
J F David, R Fernandez, B Marques, J Roucolle
  • PMID: 526623

[Growth and morphologic characteristics of normal human endometrial cells in primary culture]

[Article in French]
J F David et al. Bull Assoc Anat (Nancy). 1979 Mar.

Abstract

Monolayer cultures of endometrium were established from 59 normal women after hysterectomy. The technical procedure is described, allowing cells in monolayer to grow satisfactorily for more than 60 days, showing a doubling-time of about 4-5 days. During early passages, two cell populations are characterized by microscopic examination : confluent areas of epithelial polygonal cells and scattered fusiform fibroblastic cells. After the 8th passage, epithelial cells are no longer recognized : the monolayer is homogeneous and all cells are fibroblast-like. These cells are an interesting model system for steroid sex hormone studies.

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