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. 1970 Sep;67(1):68-73.
doi: 10.1073/pnas.67.1.68.

Polynucleotide ligase-catalyzed joining of deoxyribo-oligonucleotides on ribopolynucleotide templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates

K Kleppe, J H Van de Sande, H G Khorana

Polynucleotide ligase-catalyzed joining of deoxyribo-oligonucleotides on ribopolynucleotide templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates

K Kleppe et al. Proc Natl Acad Sci U S A. 1970 Sep.

Abstract

T4 polynucleotide ligase efficiently catalyzes the head-to-tail joining of the ribo-oligoadenylates, r-(pA)(8) and r-(pA)(10), in the presence of high molecular weight deoxypolythymidylate. The enzyme also catalyzes the joining of deoxy-oligothymidylates, e.g., d-(pT)(10), in the presence of ribopolyadenylate. The enzyme failed to bring about the joining of r-(pA)(10) when poly r-U was used as the template, although a slow formation of the expected activated intermediate from r-(pA)(10) was detected.

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References

    1. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 - PubMed
    1. J Mol Biol. 1966 Sep;20(2):359-89 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1841-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Jul;58(1):240-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 May;57(5):1426-33 - PubMed