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. 1979 Nov;64(2):189-93.
doi: 10.1007/BF00490098.

Acridine orange staining of the mammalian fibroblast cell coat

J Timár, G Gyapay, K Lapis

Acridine orange staining of the mammalian fibroblast cell coat

J Timár et al. Histochemistry. 1979 Nov.

Abstract

Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compounds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric--in vitro--measurements and enzymatic digestions, respectively.

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References

    1. Proc Natl Acad Sci U S A. 1971 May;68(5):942-5 - PubMed
    1. J Cell Biol. 1974 Oct;63(1):71-83 - PubMed
    1. Histochem J. 1977 May;9(3):267-76 - PubMed
    1. J Cell Biol. 1976 Oct;71(1):280-94 - PubMed
    1. Tissue Cell. 1977;9(2):317-34 - PubMed