Corticosteroid analysis in biological fluids by high-performance liquid chromatography
- PMID: 528595
- DOI: 10.1016/s0378-4347(00)81514-5
Corticosteroid analysis in biological fluids by high-performance liquid chromatography
Abstract
A sensitive, specific, and reproducible high-performance liquid chromatographic assay for the simultaneous determination of prednisone, prednisolone and cortisol in biological fluids was developed with dexamethasone as the internal standard. Samples are extracted with methylene chloride, washed with sodium hydroxide and then water, and chromatographed on a microparticulate silica gel column with UV detection at 254 nm. Sensitivity was greater than 15 ng for all four steroids. Specificity was supported by use of dual wave-length UV detection and/or radioimmunoassay. The assay has been applied in pharmacokinetic studies and a typical plasma concentration--time profile for the three steroids is presented for one subject who received 50 mg of prednisone.
Similar articles
-
Simultaneous determination of corticosterone, hydrocortisone, and dexamethasone in dog plasma using high performance liquid chromatography.J Pharm Sci. 1982 Jul;71(7):816-8. doi: 10.1002/jps.2600710724. J Pharm Sci. 1982. PMID: 7120070
-
Two chromatographic methods for the determination of corticosteroids in human biological fluids: pharmacokinetic applications.J Pharm Sci. 1989 Aug;78(8):660-6. doi: 10.1002/jps.2600780812. J Pharm Sci. 1989. PMID: 2674406
-
Fifteen years of operation of a high-performance liquid chromatographic assay for prednisolone, cortisol and prednisone in plasma.J Chromatogr B Biomed Appl. 1994 Aug 5;658(1):47-54. doi: 10.1016/0378-4347(94)00218-5. J Chromatogr B Biomed Appl. 1994. PMID: 7952131
-
Measurement of glucocorticoids in biological fluids.Methods Mol Biol. 2006;324:141-57. doi: 10.1385/1-59259-986-9:141. Methods Mol Biol. 2006. PMID: 16761376 Review.
-
High-performance liquid chromatographic analysis of corticosteroids.J Chromatogr B Biomed Appl. 1995 Sep 15;671(1-2):319-40. doi: 10.1016/0378-4347(95)00259-l. J Chromatogr B Biomed Appl. 1995. PMID: 8520699 Review.
Cited by
-
Stress hormone concentration in Rocky Mountain populations of the American pika (Ochotona princeps).Conserv Physiol. 2013 Oct 18;1(1):cot027. doi: 10.1093/conphys/cot027. eCollection 2013. Conserv Physiol. 2013. PMID: 27293611 Free PMC article.
-
Dose-dependent pharmacokinetics of prednisolone in normal and adrenalectomized rats.J Pharmacokinet Biopharm. 1986 Oct;14(5):453-67. doi: 10.1007/BF01059655. J Pharmacokinet Biopharm. 1986. PMID: 3806371
-
Time course of the changes in prednisolone pharmacokinetics after co-administration or discontinuation of rifampin.Eur J Clin Pharmacol. 1993;45(3):287-9. doi: 10.1007/BF00315399. Eur J Clin Pharmacol. 1993. PMID: 8276057
-
Immunohistological observations in rat kidney allografts after local steroid administration.J Exp Med. 1987 Nov 1;166(5):1205-20. doi: 10.1084/jem.166.5.1205. J Exp Med. 1987. PMID: 3119756 Free PMC article.
-
Pharmacokinetics of prednisone and its metabolite prednisolone in children with nephrotic syndrome during the active phase and in remission.Br J Clin Pharmacol. 1984 Apr;17(4):423-31. doi: 10.1111/j.1365-2125.1984.tb02367.x. Br J Clin Pharmacol. 1984. PMID: 6721988 Free PMC article.